Introducing Fiber-seq: A Breakthrough in Multiomic Sequencing


We’re excited to launch Fiber-seq — a powerful new assay that combines DNA sequence, methylated CpG detection, and chromatin accessibility in one long-read sequencing experiment.
 

What is Fiber-seq?


Fiber-seq uses a DNA methyltransferase (MTase) to label accessible adenines, effectively “stenciling” chromatin accessibility directly onto the DNA. Optimized using PacBio long-read sequencing, this assay enables simultaneous detection of:
 

  • Chromatin accessibility

  • Native DNA methylation (5mC)

  • Long stretches of DNA sequence (from a single molecule)
     

Figure 1. Fiber-seq simultaneously detects open chromatin (6mA) and DNA methylation (5mC) in human leukemia (K562) cells. ATAC-seq and CTCF CUT&RUN data are shown for comparison. Each horizontal line in the Fiber-seq panels represents a single long read, providing single-molecule data.
How does Fiber-seq work?
Figure 2. The Fiber-seq workflow. A simple incubation with the Hia5 MTase is performed before processing through standard long-read sequencing workflows.

Real-World Applications of Fiber-seq

⚕️ Perform multiomic profiling to resolve a Mendelian disorder (Vollger et al.)

🧍🐒 Reveal conserved chromatin organization in human and primate centromeres (Dubocanin et al.)
🌽 Uncover the regulatory potential of transposable elements in maize (Bubb et al.)

Apply Now for Early Access

To celebrate this launch, we’re inviting a limited group of early users to try Fiber-seq at no cost. If selected, you’ll receive:

✅ A FREE tube of Hia5 methyltransferase enzyme
✅ FREE bioinformatic analysis of your Fiber-seq data (for PacBio users — terms may apply)
✅ Early access to protocols, expert support, and product updates

To apply, simply complete a brief survey below to share your feedback.
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